EnzyPep publication list:
Omniligase-1: A powerful tool for peptide head-to-tail cyclization
Marcel Schmidt, Ana Toplak, Peter J.L.M. Quaedflieg, Hans Ippel, Gaston J.J. Richelle, Tilman M. Hackeng, Jan H. van Maarseveen and Timo Nuijens
Advanced Synthesis and Catalysis (2017), in press.
Enzyme-mediated Ligation Technologies for Peptides and Proteins
Marcel Schmidt, Ana Toplak, Peter J.L.M. Quaedflieg and Timo Nuijens
Current Opinion in Chemical Biology (2017), 38, 1-7.
Engineering a Diverse Ligase Toolbox for Peptide Segment Condensation
Timo Nuijens, Ana Toplak, Peter J. L. M. Quaedflieg, Jeroen Drenth, Wu Bian and Dick B. Janssen
Advanced Synthesis and Catalysis (2016), 358(24), 4041-4048.
Omniligase and selective Peptiligases, efficient biocatalysts for assembling linear and cyclic peptides and protein conjugates
Timo Nuijens, Ana Toplak, Mathijs B.A.C. van den Meulenreek, Marcel Schmidt, Michel Goldbach, Peter J. L. M. Quaedflieg and Dick B. Janssen
Chemistry Today (2016), 34.
Peptiligase, an Enzyme for Efficient Chemoenzymatic Peptide Synthesis and Cyclization in Water
Ana Toplak, Timo Nuijens, Peter J. L. M. Quaedflieg, Bian Wu and Dick B. Janssen
Advanced Synthesis and Catalysis (2016), 358(13), 2140-2147.
Improved solid phase synthesis of peptide carboxyamidomethyl (Cam) esters for enzymatic segment condensation
Timo Nuijens, Ana Toplak, Mathijs B.A.C. van de Meulenreek, Marcel Schmidt, Michel Goldbach, Peter J.L.M. Quaedflieg
Tetrahedron Letters (2016), 57(32), 3635-3638.
Improving the carboxyamidomethyl ester for subtilisin A-catalyzed peptide synthesis
de Beer, Roseri J. A. C.; Nuijens, Timo; Wiermans, Lotte; Quaedflieg, Peter J. L. M.; Rutjes, Floris P. J. T.
Organic & Biomolecular Chemistry (2012), 10(33), 6767-6775.
EnzyPep’s technology has been recently featured in A. Henninot, J. C. Collins, J. M. Nuss, J. Med. Chem. 2017 as the ”most impressive enzymatic approach to date” with a ”near-perfect set of attributes for peptide and protein ligation applications”.
In Torreya’s current outlook about the future of the global pharmaceutical industry EnzyPep’s ligation technology has been features as a ”major innovation in the field of peptide manufacturing”.